产品英文名称：LONZA 04-418Q X-VIVO™ 15Chemically Defined, Serum-free Hematopoietic Cell Medium

产品中文名称：X-VIVO™ 15化学成分确定的无血清造血干细胞培养基

LONZA 04-418Q X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Medium 研究背景

Lonza’s R&D efforts and collaboration with many clinical trials have provided Lonza with the tools and expertise to support the developments in adoptive immunotherapy, cancer therapy, genetic therapy and other cellular therapies. Lonza’s philosophy for the development of serum-free formulations for use in cellular therapy is to provide a nutritionally complete and balanced environment for the cells. We have not included any exogenous growth factors, artificial stimulators of cellular proliferation or undefined supplements.

The products LONZA 04-418Q X-VIVO 15 are devoid of any protein-kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin and pasteurized human transferrin. All X-VIVO™ Medium products are manufactured under current GMPs and are listed with the FDA in a product Master File. Permission to cross-reference the Master File may be obtained by contacting the Product Manager.

LONZA X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium 简介

X-VIVO™ 20 Medium was developed and optimized to support the generation of LAK cells from monocyte-depleted PBL at high density. Initial cell densities between 2.0- 3.0 x 107 cells/ml were successfully used to generate LAK cells. X-VIVO™ Medium 20 may also be used as a growth medium for PBL and TIL.

LONZA X-VIVO™ 15化学成分确定的无血清造血细胞培养基特点

? 不含血清

? 化学成份明确

? 成份包含L-谷氨酰胺，庆大霉素和酚红

? LONZA 04-418Q 最适于无血清环境中浸润淋巴细胞(TIL)增殖

X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium 使用注意事项

1. LONZA 04-418Q X-VIVO™ 15 is similar in composition to X-VIVO™ 10 and has been optimized for the proliferation of Tumor Infiltrating Lymphocytes (TIL) under serum-free conditions.

2.Please see Supplemental Information for additional hematopoietic cell applications.

3.X-VIVO™ 15, catalog number 04-418 is formulated with L-glutamine, gentamicin, and phenol red. Catalog number 04-744Q contains L-glutamine but does not contain gentamicin or phenol red.

4.X-VIVO™ 15, catalog number BE02-053Q is formulated with L-glutamine, gentamicin, recombinant transferrin, and phenol red. X-VIVO™ 15, catalog number BE02-054Q, contains L-glutamine and recombinant transferrin but does not contain gentamicin or phenol red. The recombinant transferrin replaces native transferrin.

Lonza X-VIVO™ 化学成分确定的无血清造血干细胞培养基订购信息

Catalog Number      Description                                                                                Size

LONZA 04-380Q                  X-VIVO™ 10 Medium with L-glutamine, gentamicin,                1 L

and phenol red

LONZA 04-743Q                  X-VIVO™ 10 Medium with L-glutamine; without                        1 L

gentamicin or phen red

LONZA BE04-418F             X-VIVO™ 15 Medium with L-glutamine, gentamicin,                500ml

and phenol red (Europe only)

LONZA 04-418Q                 X-VIVO™ 15 Medium with L-glutamine, gentamicin,                 1 L

and phenol red

04-744Q                 X-VIVO™ 15 Medium with L-glutamine; without                         1 L

gentamicin orphenol red

04-448Q                X-VIVO™ 20 Medium with L-glutamine, gentamicin,                  1 L

and phenol red