Cellartis Y30045 DEF-CS 500 Xeno-Free Culture Medium 无外源人多能诱导干细胞培养基，不含抗生素

英文名称：Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics
中文名称：Cellartis DEF-CS 500 无外源 iPS 培养基，不含抗生素
产品编号：HY-001466
订购货号：Y30045
生产厂家：TAKARA
规格：1 Kit

组成：

• 500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium without Antibiotics (Cat. No. Y30046；不单独销售)
• Cellartis DEF-CS 500 Xeno-Free Additives (Cat. No. Y30042)
  • 500 μl DEF-CS Xeno-Free Additive 1 (1000X)
  • 200 μl DEF-CS Xeno-Free Additive 2 (1000X)

特点：

• 完全不含人源及动物源成分（xeno-free）
• 不含抗生素
• 无需饲养层细胞
• 采用温和非酶促单细胞传代方法
• 支持高增殖率与稳定核型
• 保持iPS细胞多能性、长期自我更新及分化潜能
• 高重复性与生长稳定性

用途：适用于临床前级别人诱导多能干细胞（iPS）的扩增。

保存条件：基础培养基 2–8°C；添加剂 –20°C。

配套试剂要求（需另行购买）：

• 缓冲液：D-PBS（含Ca2?/Mg2? 与 不含Ca2?/Mg2?）
• 基底：iMatrix-511（Takara Clontech, Cat. No. T303）或 Corning Synthemax II-SC（Cat. No. 3535）
• 消化剂：Versene Solution（Life Technologies, Cat. No. 15040）

Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics is intended for the expansion of human induced pluripotent stem (iPS) cells in conditions that are completely free from human- and animal-derived components. Cells are cultured in the absence of feeder cells; instead, a xeno-free substrate such as iMatrix-511 is used. The culture protocol uses gentle, non-enzymatic single-cell passaging to ensure controlled and reproducible conditions. Cells cultured in this medium have a high-proliferation rate and stable karyotype, allowing for efficient scale-up. The combination of these features makes this medium ideal for generating preclinical-grade iPS cells for regenerative medicine studies.