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PrimeGene 重组人LR3胰岛素样生长因子-1（rHuLR3 IGF-1）
2025-12-25
产品详情
- Cas：61912-98-9
- 产品名称PrimeGene 重组人LR3胰岛素样生长因子-1（rHuLR3 IGF-1）
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- 简介
Synonyms LR3 IGF-I
SourceEscherichia coli.
Molecular Weight Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.
Size 1mg/5mg/50mg
AA Sequence
MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
Purity
>98 % by SDS-PAGE analyses.
>90 % by RP-HPLC analyses.
Biological Activity
Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105 U/mg. Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 U/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2.
Endotoxin
Less than 0.01 EU/μg of rHuLR3 IGF-1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
-12 months from date of receipt, 2 to 8 °C as supplied
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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