Cellartis DEF-CS 500 iPS无外源3D悬浮培养基

传统的2D培养系统无法支持放大培养（Scale-up）所需细胞数量级别（> 10⁹个细胞/处理）以维持细胞疗法的增长需求。3D悬浮培养是大规模生产多能细胞的可行经济有效的解决方案。尽管干细胞传统上难以适应悬浮培养，但CellArtis研究人员已经开发出一种培养体系，可以在成分确定、无饲养层和无异源物条件下实现稳定高效的生长并维持多能性。Cellartis DEF-CS 500 iPS无外源3D悬浮培养基与生物反应器系统配套，为创建大量高质量、未分化的起始材料提供了理想的方法。该培养基的配方基于2D培养基Cellartis DEF-CS 500 Xeno-Free Culture Medium，2D培养的细胞可以轻松过渡到3D以实现可靠的放大。

组份

• Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)
• 500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No. Y30046; not sold separately)
• Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)
  • 500 µl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)
  • 200 µl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)
  • 400 µl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)

自行购买试剂

• PBS Dulbecco's w/o Ca²⁺ & Mg²⁺ (D-PBS --/--)
• Accumax (Merck Millipore, Cat. No. SCR006)

可选抗生素和消化试剂

• Gentamicin (Thermo Fisher, Cat. No. 15750)
• Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)

细胞培养参数