Product Description

DermaLife K cell culture medium is optimized for culturing Human Epidermal Keratinocytes in a serum-free environment.  DemaLife K supports the growth of these cells over a period of at least 15 population doublings.

DermaLife Basal Medium contains no antimicrobials and no phenol red.

DermaLife K medium is offered in a kit format composed of DermaLife Basal Medium and associated supplements and growth factors called LifeFactors®.  With this kit, you can prepare fresh medium to enhance performance.

DermaLife Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors® are packaged in a convenient gas-impermeable pouch for easy storage.  The LifeFactors® include:

ISO-Style Performance Testing to Insure Value

DermaLife K Serum-Free Medium grows Human Epidermal Keratinocytes and Oral Keratinocytes through at least 15 population doublings at rates that meet or exceed other commercially available serum-free media while maintaining excellent cell morphology. In comparisons with other commercially-available media, DermaLife K shows superior proliferation of epidermal keratinocytes at different seeding densities.

Shown left: Human Epidermal Keratinocytes, passage 2, 5 days after inoculation with 2,500 cells/cm2 (100X).

Shown below: Human Epidermal Keratinocytes inoculated at low seeding densities in 24-well plates. Higher fluorescence represents a greater number of cells per well, and therefore, better proliferation.

 Guaranteed Consistency and Reproducible Results

Lifeline® Cell Technology manufactures products using the highest quality raw materials and incorporates ISO-style quality assurance in every production run. Exacting standards and production procedures ensure lot-to-lot consistency. Every production lot of DermaLife K Medium is extensively tested using normal human keratinocytes.

FAQ 常见问题
Q: 该套装的产品稳定性如何，反复冻融对使用有影响吗？A: 产品稳定性优良，按规定条件保存可稳定存放 12 个月。生长因子不建议反复冻融，建议根据单次用量无菌分装后冷冻保存，反复冻融可能导致活性成分降解，影响细胞培养效果。
Q: 套装的保存和运输有特殊要求吗？A: 基础培养基需 2-8℃避光保存，避免长期暴露在光照下；生长因子需 - 20℃冷冻保存。运输采用冰袋低温冷链，收到货后请立即按对应条件存放，确保各组分活性不受影响。
Q: 该套装与其他品牌同类产品相比，有哪些优势？A: 相比其他品牌，本品配方更具针对性，无血清设计适配角质细胞专属生长需求，支持细胞长期增殖且形态稳定；同时华雅思创生物现货供应，无需等待进口货期，性价比更高，还可提供一对一技术支持，解答培养过程中的相关疑问。

华雅思创生物现货供应 LifeLine 人表皮角质细胞培养套装（货号 LL-0007），套装齐全、品质可靠、发货快速，全程冷链保障产品活性，助力皮肤科研、毒理检测等实验高效推进。